Precision

in proteomics.

Fluorosequencing moves beyond genomics as the world's first single- molecule protein sequencer.

Ask more

precise questions.

Fluorosequencing goes where existing technologies cannot, empowering scientists to ask key questions that will help overcome difficult-to-treat diseases.

NEUROLOGYMeasure healthy vs pathological alpha-synuclean proteins

ONCOLOGYDetect neoantigens directly for better immune-oncology treatments

INFECTIOUS DISEASEReveal the glycosylation sites COVID19 uses to evade the immune system

LEARN ABOUT APPLICATIONS

Transform the way we manage disease.

DETECTExtremely low sample sizes & liquid biopsies

TREATBetter therapeutics and targeted drugs

TRACKMore precise tracking of disease progression

Fluorosequencing’s Sweet Spot

Fluorosequencing brings all the advantages of next- generation DNA sequencing to proteins, combining leading-edge digital quantification with high sensitivity and throughput. It is ideal for quantifying vast numbers of peptides in ultra-low sample concentrations, such as those found in tumor biopsies, or in extremely rare samples.

Predicting protein sequences from mRNA levels is imprecise and unreliable.¹

Furthermore, Mass Spectrometry is not suitable for many applications because of its low sensitivity and throughput.

1. Liu, Y., Beyer, A. & Aebersold, R.Cell165, 535–550(2016).

Our technology can accurately identify proteins in ways no other technology can.

Fluorosequencing offers the ability to sequence hundreds of millions or billions of individual protein molecules simultaneously.

Competition

RNA SEQUENCING

Indirect measurement
No PTM information
Poorly correlated quantification

MASS SPECTROMETRY

High sample requirement
Sequential processing
PTM low resolution

AFFINITY ASSAYS

Qualitative measurement
Biased identification
Variable Specificity

FLUOROSEQUENCING ADVANTAGES

Ultimate sensitivity
Ultra-low sample requirement
Massively parallel throughput
Absolute quantification
De novo PTM discovery

SINGLE-MOLECULE SENSITIVITY
Fluorosequencing is 1 million times more sensitive than mass spectrometry. Assuming a factor of at least 1000, this translates into extremely low sample amounts: the difference between needing 1/4th of a pancreas or sampling with a needle for biopsy material.
MASSIVELY PARALLEL ARCHITECTURE
Our technology achieves a scalable throughput that was previously unattainable, empowering researchers and clinicians to identify hundreds of millions or even billions of peptide molecules on a single glass slide.
ABSOLUTE QUANTIFICATION
Fluorosequencing enables researchers to compare proteins in the same sample – and across experiments – without need for external calibrants. Unlike other technologies, fluorosequencing quantifies peptides and proteins by counting molecular observations.
CHARACTERIZE HIGHLY HETEROGENEOUS SAMPLES
Our technology can discriminate peptides and proteins across a large range of heterogeneity and abundances (up to 10^6 range). Identify peptides existing in extremely diverse backgrounds, such as antigens on tumor surfaces or a low abundant phosphorylation event on a protein.

Key Concept

With help from a reference database, only a few amino acids are needed to uniquely identify a protein in a proteome.

How it Works

EXAMPLE: CATALOGING ALL THE PROTEINS OF A SINGLE CELL

1.0
The protein sample is digested, creating hundreds of millions or billions of peptides.
2.0
Select amino acids are Fluorescently labeled.
3.0
The peptides are immobilized.
4.0
Image capture establishes baseline fluorescence.
5.0
The sequence is determined by the intensity of the fluorescence.
6.0
Subsequent cycles of imaging and Edman chemistry are conducted, usually 15-20 cycles. Fluorescence is analyzed and amino acids are identified to create a fluorosequence.
7.0
The fluorosequence is matched in a database to identify which protein the peptide came from.